Product Number: ACT-300
Video tracking for Open Field - Plus Maze - Radial Arm Maze - Zero Maze - Novel Object Recognition - Conditioned Place Preference
LimeLight can track up to 4 animals at once. No special lighting is necessary, and black or white animals are tracked automatically on almost any background. Any number of stationary items can be placed in the arena without affecting tracking. Collect images at up to 30 frames per second for one animal or up to 8 frames per second when tracking 4 animals at once. Tracking is robust even at extremely low light levels. Images can be stored on the hard drive for later review and can be exported to QuickTime movies for presentation.
Analysis by Grid or Zone: For all experiments, each analysis parameter can then be exported by grid square, maze arm or custom zone. All measurements can be exported directly to an open Excel spreadsheet. Parameters include:
- Distance travelled (cm or %)
- Time spent in each region (sec or %)
- Crossings into each region
- Body length from nose to base of tail (mm), together with stretch-attend bouts
- Pointing Direction (orientation)
- Mean distance from center
Handle Hundreds of Animals Easily: LimeLight was specifically designed for high throughput experiments. Collect data from any number of animals into a single data file. Sort animals into user-specified groups; when a Batch Analysis is performed, data are reported for animals in each group, together with mean and standard deviation for the group as a whole. Analyses are designed in collaboration with our users and ar tailored to the specific needs of each type of experiment.
Extremely Flexible: Switch instantly between any of LimeLight's data collection modes (Open Field, Plus Maze, Radial Arm Maze, Zero Maze, Conditioned Place Preference, Custom maze design). Arena setup is performed with a fiew mouse clicks by moving cursors over a live video image.
Stimulus Control: LimeLight has 8 output signal for controlling experimental apparatus. The user can specify that a line be turned on when the animal enters a particular zone in the arena. Stimulus duration, delay and time-out are adjustable. The 8 output lines are provided on an easy-to-install USB interface.
Behavior Scoring: Multiple user-defined behaviors can be scored during a trial using single key strokes or mouse clicks. Or score each trial offline while replaying the video stored on the computer's hard drive.
Ordering LimeLight
LimeLight has 2 different hardware configurations. The 1-camera system can be used to record 1 to 4 animals at once. For multiple animals, each one is placed in a separate arena in one quadrant of the image. The 4-camera system is also used to record up to 4 animals, but each arena is viewed by a separate camera. The additional hardware adds to the system cost, but the 4-camera setup reduces image distortion since each arena is viewed from its center and not from a corner. In addition, it increases flexibility in the placement of arenas. With each system, trials in each arena can be started and stopped together or independently. Trials can be started from the keyboard or from remotes switches (provided at no extra charge on request).
Order WaterMaze, LimeLight and/or FreezeFrame together as a single, cost-effective system that will perform almost any behavioral task. |
1-camera system components
- CCD Camera with auto-iris zoom lens
- 1-channel image acquisition board and video cable
- Remote switch
- On-line manual
4-camera system components
- 4 CCD Cameras with auto-iris zoom lenses
- 4-channel Image acquisition board and video cables
- Remote switches
- On-line manual
System requirements:
- Desktop Pentium computer with room for 1 PCI slot
- Windows 95/98/2000/XP
- 256 MBytes of memory
- 1024x768 monitor resolution
- If trial video images are to be stored to disk, a large harddrive and fast CD burner are recommended.
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