PRODUCTS


CUSTOMER SUPPORT


TECHNICAL LIBRARY

Citations
Manuals
Tutorials

Join our mailing list!





          
You are here: Home > Actimetrics
Choose a sub category:
Activity Clock Lab for Circadian Biology
FreezeFrame Interface
Video Camera and Accessories Watermaze

Actimetrics

INTRODUCTION to ACTIMETRICS

CLOCKLAB AND CLOCKLAB ANALYSIS

FREEZEFRAME

WATERMAZE

LIMELIGHT


INTRODUCTION

Actimetrics specializes in automated systems for video-based animal behavioral research and analyses. Emphasis is placed on ease of use and the application of modern signal and image processing techniques to achieve a high level of validity and reliability. Dozens of features designed for high throughput data collection and analysis benefit large and small users alike.

CLOCKLAB AND CLOCKLAB ANALYSIS

ClockLab and ClockLab Analysis are the most sophisticated and easy- to-use system available for data for Circadian Biology studies. As the clear industry standard, ClockLab combines a point-and-click interface with state-of-the art algorithms. Embedded controls transform each graph into a powerful and interactive tool for data analysis. Measure phase shifts in under 30 seconds with our fast and simple to use ClockLab program. Applied to mice, hamsters, rats, drosophila, humans, bees, algae, Eurasian Lynx, squirrels, gopher turtles, horseshoe crabs and soil chemistry signals from the Mars Viking Lander, this system really is the most flexible available on the market.
ClockLab’s data collection program incorporates a large array of features:

    • Up to 448 channels on a desktop or laptop machine
    • USB interface for instant setup
    • Reliability with over 500,000 hours of trouble-free operation logged and thousands of publications
    • Independent Start and Stop for each channel with user-selectable file names and files can be in different directories for convenient segregation of multiple, concurrent experiments
    • Monitor almost any sensor – including running wheels and infrared motion detectors
    • Integrated light control and light recording at 1-minute precision into each data file – no need to guess about blown bulbs, accidental chamber openings or faulty, misprogrammed or inaccurate light timers! Lights are controlled in up to 96 different animal chambers independently using the simple menu and slider control panel.
    • High-density switch connections
    • Remote data access via internet to download files at any time using an FTP or Windows’ network capabilities
    • Record switches, motion detectors, Minimitter ER-4000's
    • Real-time viewing of actograms, both scaled and percentile, simply by pressing buttons to view them in sequence
    • Automatic detection of low activity levels for all channels viewed simply on a single window
    • Clock synchronization of ClockLab’s program to the computer’s internal clock to be automatically kept to date
    • Multicomputer site license provided for ClockLab Analysis, the most sophisticated and easy-to-use circadian analysis package available!

With ClockLab, simply past actograms and other plots into your presentation graphics programs like PowerPoint, Illustrator or Photoshop – each graph is completely editable.
Automated detection of activity onsets and offsets make it possible to:

  • Measure phase shifts
  • Measure mean alpha
  • Predict future activity onsets

Actograms are zoomable for easy navigation through long files, just drag on an interesting part of the actogram, zoom in, and then click to display the periodogram, FFT or activity profile for the chosen data segment – it is that simple!
Export all data and analyses to spreadsheet files. Data can be accessed in many ways – thought ClockLab, Stanford Software’s Chronobiology Kit, Actiwatch, Dataquest, Minimitter, Trikinetics and others.

(Back to top)

FREEZEFRAME

The first video-based system for fear conditioning, FreezeFrame, has become the standard in its field. Combining the sensitivity of the human observer with the objectivity and throughput of automation, FreezeFrame is able to measure minute movements of grooming, sniffing, turning and rearing. The animal is monitored 2-4 times per second for data that is far more objective and reproducible. FreezeFrame can also be utilized for learned helplessness experiments, including tail suspension test and the Porsolt Forced Swim Test.
FreezeFrame is not a tracking system – featuring a proprietary motion detection algorithm, the system filters out shadows, light flicker, and camera noise to detect movements as small as 1 mm. FreezeFrame offers superior performance in low-light and is fully validated, with greater than 90% concordance with trained human observers.


Easily store and recall multiple stimulus protocols for training and testing phases. Video images can be stored for future review and also exported as QuickTime and AVI movies for presentation.
Batch export of data and analyses to Excel allows for easy analyzing of % freezing, number of bouts, bout duration and intervals for specified periods.

Either white noise or pure tone can be delivered through the computer’s sound card. The FreezeFrame system accommodates multiple sounds of different amplitude, frequency and quality throughout a single trial. Stimuli – including lights, external sound generators and/or shock generators are also controlled though the computer.

(Back to top)

WATERMAZE

WaterMaze is a unique, video-based tracking system for the Morris Water Maze, designed in collaboration with Dr. Richard Morris which incorporates a novel Project Manager for designing and executing complex experiments with multiple animals, starting points and platform positions. Once the Project parameters are specified by the user, the program specifies each day which animal to run next and which platform, allowing the most complex experiments to be run accurately and efficiently even at the highest throughput trials.

Using state-of-the-art image processing software and video-capture card, WaterMaze can acquire images up to 30 frames per second. The system identifies whether the animal is lighter or darker than the background and an algorithm screens out shadows and lighting changes during the trial. The path of travel is displayed in real time as the trial proceeds. The tracking window, shown below, uses a yellow cursor to show current position and yellow dots to depict the path.

WaterMaze offers superior analysis capabilities. Easily view for single or multiple trials and transfer directly from the program to Excel. Selectable animal and trial lists allow for easy scanning of data. Parameters reported in the program are thigmotaxis, Moser’s zones, Whishaw’s corridor, Gallagher’s proximity, quadrant times and crossings and more. Swim paths can be exported to standard drawing programs and movies of each trial can be stored and viewed in the WaterMaze Analysis window or exported to PowerPoint.

(Back to top)

LIMELIGHT

Developed in close collaboration with behavioral neuroscientists, LimeLight is a flexible and powerful video tracking system for behavioral experiments. Ideal for open field, plus maze, radial arm maze, zero maze, novel object recognition and conditioned place preference, LimeLight is easily adapted to meet the needs of a range of applications. With a complete range of features, our system is very easy to configure, operate and acquire meaningful data.
LimeLight easily tracks up to four arenas simultaneously. A single camera can be used for multiple arenas or for greater flexibility; one camera can be used for each arena. Powerful enough to track white or black animals on any background, special lighting is not necessary. Images are collected up to 30 frames per second for a single animal or up to eight frames per second when tracking four arenas at once. Images are compressed and stored for later review and off-line scoring of complex behaviors. Video can easily be exported to AVI or QuickTime format for inclusion in presentations.
Our system simplifies zone creation through dedicated interfaces for open field and elevated plus maze testing. A custom zone setup permits unlimited flexibility for more specialized applications with an easy-to-use click-and-drag interface. Zone configurations can be edited during analysis and applied to previously recorded trials.
Scored behaviors can be noted by assigning a key stroke or by mouse clicking either during the trial or viewing stored video. Statistics on scored behaviors can be viewed and exported to Excel for analysis.
A main analysis window provides a graphic overview for each trial. Acquired data can be exported zone-by-zone or as a summary and are both displayed in Excel as well as saved to a text file. Data from a group of animals can be collected into a single data file and later sorted. Analysis is extremely flexible and parameters include:

  • Distance travelled in each region (cm or %)
  • Time spent in each region (sec or %)
  • Crossings into each region
  • Body length from nose to base tail (mm), together with stretch-attend bouts
  • Pointing direction (orientation)
  • Mean distance from center
  • Latency to zone
  • Distance and velocity vs. time
  • Statistics for scored behaviors (1st and 2nd order)
  • Statistics on occurrence of stimuli (output control)
  • Zone sequence
  • Circling

(Back to top)


CLOCKLAB ADDITIONAL 56-CHANNEL INTERFACE (448 MAXIMUM CHANNELS)
CLOCKLAB ADDITIONAL 56-CHANNEL INTERFACE (448 MAXIMUM CHANNELS)
Contact our Behavioral Research Specialists for more information

ClockLab's 56-channel interface is a small (8" wide) box that connects to the computer via a USB cable. Using a USB hub, up to 8 interface boxes can be connected to a single computer, for 448 input channels.

Each interface box has on its face 8 RJ-45, or ethernet-style, connectors. These connectors have 8 pins. As shown in the diagram below, one pin serves as a ground, and the other as inputs for 7 different channels. Each switch (or other input device, such as an infrared motion detector) is simply connected between the ground and one of the signal pins. When the switch closes, the program detects an event on the corresponding input channel.

Each input channel can be used to count events on a switch or other detector. Or it can be configured to detect the lights in the experimental room or chamber. For light detection, a photodetector (provided) is connected to the input channel, and the program is configured to store that channel's data as the light cycle for a specified set of counting channels.

Also availalbe are simple breakout boxes that convert each RJ-45 input to 7 pairs of banana jacks for direct connection to switches and light detectors.
7-CHANNEL BREAKOUT BOX FOR CLOCKLAB
7-CHANNEL BREAKOUT BOX FOR CLOCKLAB
Contact our Behavioral Research Specialists for more information

Used to convert each RJ-45 input to 7 pairs of banana jacks for direct connection to switches and light detectors.
4-CHANNEL STIMULUS LIGHT EXPANSION
4-CHANNEL STIMULUS LIGHT EXPANSION
Contact our Behavioral Research Specialists for more information

Converts one of the two RJ-45 rear panel low voltage outputs on the ACT-556 or -556A into four separate and controllable BNC outputs.
4-CHANNEL STIMULUS BREAKOUT BOX
4-CHANNEL STIMULUS BREAKOUT BOX
Contact our Behavioral Research Specialists for more information

Converts one of the two RJ-45 rear panel low voltage outputs on the ACT-556 or -556A into four separate and controllable 28VDC AUX connections used by Coulbourn modules.
FREEZEFRAME 4 SOFTWARE
FREEZEFRAME 4 SOFTWARE
Contact our Behavioral Research Specialists for more information

FreezeFrame has become the standard in the field for automated fear conditioning. It combines the sensitivity of the human observer with the objectivity and high throughput of automation. While infrared beams can detect gross movements of an animal from one part of the cage to another, FreezeFrame can detect the minute movements of grooming, sniffing, turning and rearing. And FreezeFrame monitors the animal 2-4 times per second, not once every 5 seconds, for far more objective and reproducible results.

Not a tracking system. A proprietary motion detection algorithm filters out shadows, light flicker and camera noise, and detects movements as small as 1 mm.

Fully validated. Greater than 90% concordance between FreezeFrame and trained human observers.

Ordering FreezeFrame:

Number of channels:

The basic 4-channel system uses one computer to record from up to 4 animals at once. 4 cameras are provided, with each camera viewing one animal. For 8- and 12-channel operation, 2 or 3 computers can be connected together via ethernet or serial cables. The user then interacts with only one computer, which in turn controls the others.

Order WaterMaze, LimeLight and/or FreezeFrame together as a single, cost-effective system that will perform almost any behavioral task.  
4 CCD cameras with auto-iris zoom lenses
4-channel video acquisition board
Cables and connectors
Interface box for shocker control outputs
Data acquisition and analysis software
System requirements:

Desktop Pentium computer with room for 1 PCI slot
Windows 95/98/2000/XP
256 MBytes of memory
1024x768 monitor resolution
If trial video images are to be stored to disk, a large harddrive and fast CD burner are recommended.
FREEZEFRAME SHOCK CONTROL EXPANSION KIT
FREEZEFRAME SHOCK CONTROL EXPANSION KIT
Contact our Behavioral Research Specialists for more information

The ACT-110 includes the connectors and cabling for controlling up to four shockers in a FreezeFrame Fear Conditioning System.  One ACT-110 is required for each FreezeFrame system
FREEZEFRAME LIGHT/TONE CONTROL EXPANSION KIT
FREEZEFRAME LIGHT/TONE CONTROL EXPANSION KIT
Contact our Behavioral Research Specialists for more information

The ACT-120 includes the connectors and cabling for controlling up to four light stimuli in a FreezeFrame Fear Conditioning System.  One ACT-120 is required for each FreezeFrame system that employs a visual stimulus.
FREEZEFRAME FAN/HOUSE LIGHT CONTROL KIT
FREEZEFRAME FAN/HOUSE LIGHT CONTROL KIT
Contact our Behavioral Research Specialists for more information

The ACT-130 includes the connectors and cabling for operating up to four House Lights and Isolation Cubicle Fans in a FreezeFrame Fear Conditioning System. One ACT-130 is required for each FreezeFrame system that employs House Lights and/or Isolation Cubicle Fans.

This devices is available in two versions:
  • ACT-130 -> FREEZEFRAME FAN/HOUSE LT. KIT 110V
  • ACT-130-220 -> FREEZEFRAME FAN/HOUSE LT. KIT 220V
ADJUSTABLE WATERMAZE PLATFORM
ADJUSTABLE WATERMAZE PLATFORM
Contact our Behavioral Research Specialists for more information

The ACT-202 is an adjustable Water Maze platform.

The dimensions are:
Base Plate: 14 inches x 14 inches (35.56 cm x 35.56 cm)
Height: Adjustable from 12 inches to 20 inches (30.48 cm to 50.80 cm)
Disc Diameters: 3 inches, 6 inches and 9 inches (7.62 cm, 15.24 cm and 22.86 cm)
CLOCKLAB SOFTWARE FOR CIRCADIAN BIOLOGY
CLOCKLAB SOFTWARE FOR CIRCADIAN BIOLOGY
Contact our Behavioral Research Specialists for more information

Data Analysis and Collection for Circadian Biology

Does it take you longer than 30 seconds to measure phase shifts? If so, ClockLab, the industry standard for the collection and analysis of circadian activity data, can help. It combines a point-and-click interface with state-of-the-art algorithms. Embedded controls turn each graph into a powerful interactive tool for data analysis. Our users enjoy ClockLab's speed and convenience. ClockLab's Analysis program has been applied to mice, hamsters, rats, drosophila, humans, bees, algae, Eurasion Lynx in the zoo, radio-tagged squirrels in the wilds of Alaska, horseshoe crabs, and soil chemistry signals from the Mars Viking Lander.

ClockLab's data collection program incorporates a large array of features at reasonable cost:
    Up to 448 channels on a desktop or laptop machine
    USB interface for instant setup
    Integrated light control and light recording
    High-density switch connections
    Remote data access via internet
    Record switches, motion detectors, Minimitter ER-4000's
Paste actograms and other plots into presentation graphics programs like PowerPoint, Illustrator or Photoshop. Every graph is completely editable.
Automated detection of activity onsets and offsets makes it possible to
    Measure phase shifts
    Measure mean alpha
    Predict future activity onsets... all with a few mouse clicks.
Zoomable actograms for easy navigation through long records. Just drag on an interesting part of the actogram to zoom in. Then click to display the periodogram, FFT or activity profile for the chosen data segment.
Chi-squared periodograms, activity profiles, FFT's and auto-correlograms.
Bout anlaysis.
Export all data and analyses to spreadsheet files.
Access data in many formats, including...ClockLab format, Stanford Software's Chronobiology Kit, Actiwatch, Dataquest, Minimitter, Trikinetics and others.
Extensive batch processing capabilities for printing and analysis.
Windows compatible.

Analysis program (Circadian Toolbox)

A single-computer license for the analysis program can be purchased on its own. It requires the purchase of Matlab (The Mathworks, Natick, MA). Only the basic Matlab is required. Toolboxes, simulink, the compilers and Excel Builder are not required. The Student verison is not recommended and will not work for large data files.

Complete system (Data collection and analysis)

56 Channels
1 56-channel interface box

112 Channels
2 56-channel interface box

168 Channels
3 56-channel interface box

224 Channels
4 56-channel interface boxes

Light-control hardware
8 channels per interface. Up to 100 additional channels available with special purpose output hardware

Minimitter E-Mitter recording module.
Hardware available separately from Mini-Mitter. Ordered as add-on to 56 or more channel-system.

Preconfigured computer
Preloaded software, data acquisition boards and drivers (Monitor not included).

Complete system setup
We will help identify and install chambers, cages, wheels and other sensors.


Multi-computer site license for the ClockLab Analysis program is available with each data collection system.

Upgrades of the data collection and analysis program are free for 3 years.

System Requirements

The data analysis system runs on Windows 2000/XP and requires only the basic MATLAB program (no toolboxes or Simulink necessary) from The MathWorks, Inc. if your institution does not already have a site license available.

Data collection requires a computer running Windows 2000/XP with a clock speed of 500 MHz or greater and 128 MB memory. Monitor resolution of 1024x768 or better. Zip drive , flash memory stick or network connection recommended for accessing files during data collection.